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| Registros recuperados : 49 | |
| 4. |  | COTTA, M. G.; MARRACCINI, P.; REGO, E. C. S.; SIDIBE-BOCS, S.; CARNEIRO, F. A.; THIS, D.; ANDRADE, A. C. First step of ABA-mediated response to drought in Coffea. In: In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls, Brazil. Book of Abstracts... Rockville, USA: American Society of Plant Biologists, 2015. p. 0881.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | COTTA, M. G.; VERAS, H. C. T.; BARROS, L. M. G.; ABDELNOOR, R. V.; ALMEIDA, J. D.; SILVA, F. R. Finding novel tissue-specific genes using the new publically available soybean est database. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 86.| Biblioteca(s): Embrapa Soja. |
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| 6. | | SOUSA, H. R.; COTTA, M. G.; RODRIGUES, G. C.; ALMEIDA, J. D.; CARNEIRO, M.; BARROS, L. M. G. Isolamento de RNA de diferentes órgãos de Coffea arabica adequado para ensaios de expressão gênica. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 045. p. 85.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | COTTA, M. G.; BARROS, L. M. G.; ALMEIDA, J. D. de; ANDRADE, A. C.; CARNEIRO, M.; MARRACCINI, P. Validation of coffee seed promoter in nicotiana tabacum. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 256.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | COSTA, T. S.; CARNEIRO, F. A.; COTTA, M. G.; LIMA, E. A.; RÊGO, E. C. S.; MARRACCINI, P.; ANDRADE, A. C. Analysis of the expression of mannose-6-phosphate reductase gene in roots of different clones of C. canephora subbmited to water deficit. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016. p. 186.| Biblioteca(s): Embrapa Café. |
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| 9. | | BARROS, L. M. G.; ANDRADE, L. R. M. de; GEEST, J. J. Van Der; COTTA, M. G.; RIBEIRO, D. G.; FRANCO, A. C.; BECQUER, T. Acumulação de alumínio em teceidos foliares de espécies nativas do Cerrado. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 11., 2007, Gramado, RS. Fotossíntese: a essência da vida: [Resumos...]. Pelotas, RS: SBFV, 2007. Publicado também em: Brazilian Journal of Plant Physiology, v. 19, supl., 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | BARROS, L. M. G.; ANDRADE, L. R. M. de; GEEST, J. J. Van D.; COTTA, M. G.; RIBEIRO, D. G.; FRANCO, A. C.; BECQUER, T. Acumulação de alumínio em tecidos foliares de espécies nativas do Cerrado. Brazilian Journal of Plant Physiology, Campinas, v. 19, 2007. Suplemento. 1 CD-ROM. Edição dos Resumos do XI Congresso Brasileiro de Fisiologia Vegetal, Gramado, set. 2007.| Biblioteca(s): Embrapa Cerrados. |
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| 11. | | ANDRADE, L. R. M. de; FRANCO, A. C.; HARIDASAN, M.; BARROS, L. M. G.; GEEST, J. J. Van D.; COTTA, M. G.; RIBEIRO, D. G. Alumínio nos ápices radiculares de espécies nativas do Cerrado. Brazilian Journal of Plant Physiology, Campinas, v. 19, 2007. Suplemento. 1 CD-ROM. Edição dos Resumos do XI Congresso Brasileiro de Fisiologia Vegetal, Gramado, set. 2007.| Biblioteca(s): Embrapa Cerrados. |
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| 12. | | ANDRADE, L. R. M. de; FRANCO, A. C.; HARIDASAN, M.; BARROS, L. M. G.; GEEST, J. J. Van Der; COTTA, M. G.; RIBEIRO, D. G. Alumínio nos ápices radiculares de espécies nativas do Cerrado. In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 11., 2007, Gramado, RS. Fotossíntese: a essência da vida: [Resumos...]. Pelotas, RS: SBFV, 2007. Publicado também em: Brazilian Journal of Plant Physiology, v. 19, supl., 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | COTTA, M. G.; RÊGO, E. C. S.; BOCS, S. S.; COSTA, T. S.; DUFAYARD, J-F.; THIS, DOMINIQUE; MARRACCNINI, P.; ANDRADE, A. C. The first step of ABA perception and signal transduction in coffe: evolutionary and expressionof PYR/PYL/RCARs, PP2Cs and SnRK2s genes in C canephora under drought. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016.| Biblioteca(s): Embrapa Café. |
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| 15. | | COTTA, M. G.; VERAS, H C. T.; BARROS, L. M. G.; ABDELNOOR, R. V.; ALMEIDA, J. D. D.; SILVA, F. R. de. Finding novel tissue-specific genes using the new publically available soybean est database. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 86.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | COSTA, T. S.; MELO, J. A. T.; CARNEIRO, F. A.; VIEIRA, N. G.; RÊGO, E. C. S.; LIMA, E. A.; COTTA, M. G.; MARRACCINI, P.; ANDRADE, A. C. Expression of candidates genes for drought tolerance related to ABA signaling in roots of C. Canephora. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 6., 2015, Brasília. [Resumos]. Brasília, DF: Sociedade Brasileira de Biotecnologia, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | COTTA, M. G.; BOCS, S.; REGO, E. C. S.; COSTA, T. S.; AQUINO, S. O. de; CARNEIRO, F. De A.; THIS, D.; MARRACCINI, P.; ANDRADE, A. C. Estudo das famílias gênicas CcPYL, CcPP2C e CcSnRK2: componentes centrais na via aba-dependente para a resposta ao déficit hídrico em Coffea Canephora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., Curitiba. Brasília, DF: Embrapa Café, 2015.| Biblioteca(s): Embrapa Café. |
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| 18. | | LIMA, E. A. de; REGO, E. C. S.; COTTA, M. G.; COSTA, T. S.; CARNEIRO, F. A.; CARNEIRO, R. M. D. G.; MARRACCINI, P.; ANDRADE, A. C. Differential expression of candidate genes to resistance Tomeloidogyne paranaensis in clones of Coffea canephora. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016. p. 185.| Biblioteca(s): Embrapa Café. |
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| 19. | | GEEST, J. J. V. D.; COTTA, M. G.; ANDRADE, L. R. M. de; BARROS, L. M. G.; ALMEIDA, J. D. de; FRANCO, A. C. Desenvolvimento de metodologia para detecção de alumínio nos tecidos de plantas do cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 3., 2007, Planaltina, DF. Resumos apresentados. Planaltina, DF: Embrapa Cerrados, 2007. p. 15. (Embrapa Cerrados. Documentos, 176).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | GEEST, J. J. V. D.; COTTA, M. G.; ANDRADE, L. R. M. de; BARROS, L. M. G.; ALMEIDA, J. D. de; FRANCO, A. C. Desenvolvimento de metodologia para detecção de alumínio nos tecidos de plantas do cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 3., 2007, Planaltina, DF. Resumos apresentados. Planaltina, DF: Embrapa Cerrados, 2007. p. 15. (Embrapa Cerrados. Documentos, 176).| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 49 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
22/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
COTTA, M. G.; MARRACCINI, P.; THIS, D.; LEROY, T.; BOCS, S.; DROC, G.; DUFAYARD, J. F.; DEREEPER, A.; LASHERMES, P.; ANDRADE, A. C. |
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Afiliação: |
M. G. COTTA, UFLA; P. MARRACCINI, CIRAD UMR AGAP; D. THIS, CIRAD UMR AGAP; T. LEROY, CIRAD UMR AGAP; S. BOCS, CIRAD UMR AGAP; G. DROC, CIRAD UMR AGAP; J. F. DUFAYARD, CIRAD UMR AGAP; A. DEREEPER, IRD UMR RPM; P. LASHERMES, IRD UMR RPM; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Molecular mechanisms in the first step of ABA-mediated response to drought in Coffea canephora clones. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-50. |
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Idioma: |
Inglês |
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Conteúdo: |
Global climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subclasses and subfamilies. The gene structures of these three gene families were functionally annotated in Coffee Genome Hub (http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones of C. canephora. By comparing gene expression profiles of only drought tolerant (Cc14, Cc73 and Cc120) clones, differential responses were observed suggesting the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs. MenosGlobal climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subc... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
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Marc: |
LEADER 03092nam a2200229 a 4500
001 2041744
005 2023-03-22
008 2015 bl uuuu u00u1 u #d
100 1 $aCOTTA, M. G.
245 $aMolecular mechanisms in the first step of ABA-mediated response to drought in Coffea canephora clones.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-50.$c2015
520 $aGlobal climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subclasses and subfamilies. The gene structures of these three gene families were functionally annotated in Coffee Genome Hub (http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones of C. canephora. By comparing gene expression profiles of only drought tolerant (Cc14, Cc73 and Cc120) clones, differential responses were observed suggesting the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs.
653 $aCoffee
700 1 $aMARRACCINI, P.
700 1 $aTHIS, D.
700 1 $aLEROY, T.
700 1 $aBOCS, S.
700 1 $aDROC, G.
700 1 $aDUFAYARD, J. F.
700 1 $aDEREEPER, A.
700 1 $aLASHERMES, P.
700 1 $aANDRADE, A. C.
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